Cyclosporin A induced toxicity in mouse liver slices is only slightly aggravated by Fxr-deficiency and co-occurs with upregulation of pro-inflammatory genes and downregulation of genes involved in mitochondrial functions

Table 3 GO analysis of genes uniquely regulated in WT-PCLS or FXRKO-PCLS

	General process	GO process (WT-PCLS)	FDR	Direction of change
WT-PCLS	ECM	GO:0031012 ~ extracellular matrix	9.4E-10	Downregulation
	ECM	GO:0005578 ~ proteinaceous extracellular matrix	2.0E-09	Downregulation
	ECM	GO:0044421 ~ extracellular region part	1.5E-06	Downregulation
	ECM	GO:0005576 ~ extracellular region	2.0E-04	Downregulation
FXRKO-PCLS	Inflammation	GO:0005125 ~ cytokine activity	6.1E-05	Upregulation
	Inflammation	GO:0005615 ~ extracellular space	4.6E-03	Upregulation
	Inflammation	GO:0006935 ~ chemotaxis	9.8E-03	Upregulation
	Inflammation	GO:0042330 ~ taxis	9.8E-03	Upregulation
	Mitochondrial functions	GO:0005739 ~ mitochondrion	2.0E-11	Downregulation
	Mitochondrial functions	GO:0048037 ~ cofactor binding	1.5E-03	Downregulation
	Mitochondrial functions	GO:0044429 ~ mitochondrial part	2.9E-03	Downregulation

GO analysis of the significant genes (FDR ≤ 0.05) uniquely regulated by CsA in WT-PCLS or FXRKO-PCLS. GO analysis was performed in DAVID and GO process was considered as significant if FDR ≤ 0.005. In addition, the GO processes were grouped according to their general function and these groups are depicted in column denoted as “General function”. The last column informs whether a GO process was up- or down-regulated (“Direction of change”)

ISSN: 1471-2164