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Fig. 4 | BMC Genomics

Fig. 4

From: Polymorphisms in early neurodevelopmental genes affect natural variation in alcohol sensitivity in adult drosophila

Fig. 4

Functional confirmations of candidate genes. a Candidate genes from the DGRP, extreme QTL GWA analyses and the genetic network analysis were functionally tested using transposon insertion mutations and RNAi knockdown lines. All 16 candidate genes showed significant differences from the control for at least one of the genetic terms (G, G × S, G × E, G × S × E). G – Genotype: mutant or control; S – Sex: Male, Female; E − Exposure: E1, E2; * - genes present in genetic network from Fig. 3; # – missing gene from the genetic network. M_E1, M_E2, F_E1 and F_E2 – phenotypic effects in males (M) and females (F), respectively after an acute (E1) or repeated (E2) ethanol exposures. b Effects of 16 mutants of candidate genes on sensitivity to acute (top panel) and repeated (bottom panel) ethanol exposure in males (left) and females (right) compared to control. Squares indicate candidate genes found only in GWA analyses; circles indicate candidate genes found only from extreme QTL mapping analyses; Triangles indicate missing genes from the genetic network in Fig. 3. Candidate genes found in more than one analyses (GWA, extreme QTL mapping and network analysis) are shown with diamond shapes. Data are presented as the deviation of the MET of each line and sex from the appropriate control line ± SEM, calculated as \( \sqrt{SE{m}^2+SE{c}^2} \), where m and c are SE for mutant and control lines, respectively. The color bar indicates the significance levels for both Fig. 4a and b

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