Table 2 Validation of variants with a read frequency ranging between 20 and 100Â % using targeted PCR and sanger sequencing
Genea | Sanger chromatogram result | Read frequency (%) | Sanger result |
|---|---|---|---|
Rv2316 | Single peak | 127/127 (100.0) | True |
Rv1703c | Single peak | 152/154 (98.7) | True |
Rv0820 | Single peak | 193/200 (96.5) | True |
Rv3220c | Single peak | 129/134 (96.3) | True |
Rv0537c | Single peak | 98/126 (77.8) | True |
Rv2692 | Single peak | 102/142 (71.8) | True |
Rv1521 | Double peaks | 157/222 (70.7) | True |
Rv0521 | Double peaks | 117/159 (68.8) | True |
Rv1904 | Double peaks | 119/181 (65.7) | True |
Rv1230c | Double peaks | 96/155 (61.9) | True |
Rv3086 | Double peaks | 84/145 (57.9) | True |
Rv3083 | Double peaks | 55/95 (57.9) | True |
Rv1429 | Double peaks | 74/129 (57.4) | True |
Rv2577 | Double peaks | 33/61 (54.1) | True |
Intergenic (1093238) | Double peaks | 89/168 (53.0) | True |
Rv3391 | Double peaks | 57/113 (50.4) | True |
Rv2689c | Double peaks | 59/123 (48.0) | True |
Rv0970 | Double peaks | 48/104 (46.2) | True |
Rv2173 | Double peaks | 54/130 (41.5) | True |
Rv1929c | Double peaks | 52/128 (40.6) | True |
Rv2459 | Double peaks | 18/45 (40.0) | True |
Rv2984 | Double peaks | 34/89 (38.2) | True |
Rv1894c | Double peaks | 36/95 (37.9) | True |
Rv1316c | Double peaks | 72/198 (36.4) | True |
Rv1021 | Double peaks | 42/119 (35.3) | True |
Rv2544 | Double peaks | 50/151 (33.1) | True |
Rv3772 | Double peaks | 47/147 (32.7) | True |
Rv3861 | Double peaks | 38/119 (31.9) | True |
Rv3780 | Double peaks | 30/106 (31.9) | True |
Rv0491 | Double peaks | 44/138 (31.9) | True |
Rv2957 | Single peak | 47/154 (30.5) | False |
Rv1549 | Double peaks | 42/138 (30.4) | True |
Rv3703c | Single peak | 37/126 (29.4) | False |
Rv0594 | Double peaks | 61/202 (29.2) | True |
Rv0372c | Single peak | 85/296 (28.7) | False |
Rv1479 | Single peak | 34/120 (28.3) | False |
Rv0780 | Single peak | 44/159 (27.7) | False |
Rv0092 | Double peaks | 47/170 (27.6) | True |
Rv0688 | Double peaks | 56/209 (26.8) | True |
Rv0282 | Single peak | 54/205 (26.3) | False |
Rv0663 | Double peaks | 41/158 (25.9) | True |
Rv0522 | Double peaks | 66/263 (25.1) | True |
Rv1660 | Double peaks | 52/239 (21.8) | True |
Rv1627c | Single peak | 34/161 (21.1) | False |
Rv0654 | Double peaks | 34/167 (20.4) | True |
Rv2934 | Double peaks | 47/232 (20.3) | True |