Fig. 11

Expression-constrained metabolic modeling. a In silico model predictions of the potential capacity of PAO1 and PAHM4 to produce various virulence factors of interest when grown in rich medium. Capacity units correlate to the maximum flux through an objective reaction representing the production of each factor normalized by the max uptake flux through the limited carbon uptake reactions for the optimization. b In silico estimation of biomass yield during growth in minimal media conditions supplemented with a single carbon source (amino acids and a selection of sugars as shown). Presented values are flux through the optimized biomass function normalized by flux through the single carbon source uptake reaction