Fig. 3
Protease and elastase assays. a PAHM4, PAO1, and PA14 were grown overnight in LB and filtered culture supernatants were added to an azocasein solution. After incubation, trichloroacetic acid was added to precipitate the remaining proteins and the OD495 was measured. Data presented are the OD495/OD600 of the overnight culture. b Elastase assays were performed by adding the filtered culture supernatant to an elastin-congo red solution and measuring the OD400 after 2 h. All assays were performed in triplicate, and the mean is plotted with error bars representing the standard deviation. Based on Tukey’s multiple comparison test: *, p = 0.0217,**, p = 0.0085, ****, p = < 0.0001