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Table 3 RT-qPCR expression levels of antibiotic determinants

From: Genotypic and phenotypic analyses of a Pseudomonas aeruginosa chronic bronchiectasis isolate reveal differences from cystic fibrosis and laboratory strains

 

PAO1

HM4

 

Basal Expression

Induced expressiona

Basal Expression

Induced expressiona

ampC

1

329 ± 8

509 ± 4

512 ± 181

creD

1

32 ± 12

32 ± 13

25 ± 7

mexB

1

 

0.006 ± 0.002

 

mexD

1

 

0.007 ± 0.001

 

mexF

1

 

2.1 ± 0.2

 

mexY

1

 

30 ± 11

 
  1. Mean values of relative (compared to PAO1) mRNA levels obtained in at least three independent duplicate experiments. aFor ampC and creD induction experiments, cultures were incubated in the presence of 50 μg/mL cefoxitin. Cefoxitin is an inducer of AmpC (and CreD) expression but does not affect efflux pump (mexBDFY) expression
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BMC Genomics

ISSN: 1471-2164

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