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Table 3 RT-qPCR expression levels of antibiotic determinants

From: Genotypic and phenotypic analyses of a Pseudomonas aeruginosa chronic bronchiectasis isolate reveal differences from cystic fibrosis and laboratory strains

  PAO1 HM4
  Basal Expression Induced expressiona Basal Expression Induced expressiona
ampC 1 329 ± 8 509 ± 4 512 ± 181
creD 1 32 ± 12 32 ± 13 25 ± 7
mexB 1   0.006 ± 0.002  
mexD 1   0.007 ± 0.001  
mexF 1   2.1 ± 0.2  
mexY 1   30 ± 11  
  1. Mean values of relative (compared to PAO1) mRNA levels obtained in at least three independent duplicate experiments. aFor ampC and creD induction experiments, cultures were incubated in the presence of 50 μg/mL cefoxitin. Cefoxitin is an inducer of AmpC (and CreD) expression but does not affect efflux pump (mexBDFY) expression