Fig. 3

Expression patterns driven by zebrafish and mouse syntenic ECRs with homologous activity in a zebrafish transgenic enhancer assay. ECRs are identified by the number at the bottom left of each panel, their genomic coordinates are in Table 4. Expression patterns were recorded at 24 hpf (ECR 7) or 48 hpf (ECRs 6, 10, 11, and 12) and detected by GFP expression (green). Labels indicate tissues in which homologous mouse and zebrafish sequences show consistent patterns of GFP expression (see Experimental Procedures for the definition of consistent pattern of expression). ECR 6: constructs from both species drive strong expression in several epidermal cells; the zebrafish construct also drives expression in the otic vesicle , weak staining at the periphery of the vesicle is visible with the mouse construct (see also Additional file 1: Figure S1). ECR 7: the mouse construct drives expression in the olfactory bulb, but expression by the zebrafish construct is restricted to the olfactory epithelium (the sensory component of the olfactory bulb – see also Additional file 1: Figure S1); constructs from both species drive expression in large neurons in the hindbrain of. ECR 10 and 11: one ECR is present in the mouse, and two ECRs are present in the orthologous zebrafish intron; constructs from both species drive clear expression in somitic muscle cells. ECR12: constructs from both species drive clear expression in several epidermal cells. Abbreviations – e: epidermis; ov: otic vesicle; oe: olfactory epithelium; ob: olfactory bulb; hb: hindbrain; sm: somitic muscle