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Table 4 Zebrafish and mouse predicted enhancers subjected to experimental validation

From: Functionally conserved enhancers with divergent sequences in distant vertebrates

ECR

Zebrafish (Zv9 July 2010)

Mouse (mm9) July 2007)

Expression

Coordinates

Expression

Coordinates

1

Spinal cord (48)

chr6:20,797,116-20,798,106

negative

chr5:107,545,775–107,546,750

2

Epidermis (24,48)

chr6:43,538,446–43,539,094

negative

chr6:98,881,656–98,882,625

negative

chr6:98,883,956–98,884,889

3

Tail bud (24), eye (48), heart (48)

chr9:11,657,708–11,658,704

not tested

 

4

Notochord (24,48)

chr19:22,713,465–22,714,026

negative

chr18:80,876,694–80,877,675

negative

chr18:80,876,778–80,877,739

5

Epidermis (24,48)

chr19:41,272,614–41,273,241

not tested

 

6

Epidermis (24,48), Otic Vesicle (24,48), Somitic muscle (24,48)

chr2:39,790,777–39,791,782

Epidermis (24,48), Notochord (24), Olfactory epithelium (24,48), Spinal cord (24), Otic Vesicle (48), Pectoral fin (48)

chr1:77,387,974–77,389,004

7

Hindbrain (24,48), Olfactory epithelium (24,48)

chr2:39,792,312–39,793,241

Eye (24,48), Olfactory bulb (24,48), Hindbrain (24,48)

chr1:77,390,034–77,391,031

8

Somitic muscle (24,48), Olfactory epithelium (48), Hindbrain (48)

chr2:39,837,096–39,838,009

negative

chr1:77,503,689–77,504,676

negative

chr1:77,506,344–77,507,445

9

Spinal cord (24,48)

chr4:16,470,975–16,471,819

Midbrain (24), Yolk (24)

chr10:87,004,979–87,005,964

Somitic Muscle (24,48), Skin under yolk (24,48), Phar. Arches (48), Pectoral fin (48)

chr10:87,008,830–87,009,792

10

Somitic muscle (24,48), Olfactory epithelium (48)

chr9:22,365,926–22,366,799

Somitic Muscle (24,48), Pericardium (24,48)

chr14:58,234,894–58,235,874

11

Hindbrain (24), Somitic muscle (24,48), Heart (48)

chr9:22,368,460–22,369,405

12

Epidermis (24,48), Otic vesicle (24,48), Yolk (24,48)

chr14:41,645,428–41,646,386

Epidermis (24), Olfactory epithelium (24)

chrX:130,162,955–130,163,923

13

Hindbrain (24), Olfactory epithelium (24,48), Line under eye (24,48), Spinal cord (48)

chr20:21,970,078–21,970,911

Spinal cord (24)

chr12:72,999,373–73,000,279

  1. Tissues in bold (in ECR 6, 7, 10, 11, 12) show consistent enhancer activity between the mouse and zebrafish syntenic enhancers at the indicated time point (24 or 48 hpf). Each construct was injected in at least 100 embryos; the proportion of fish alive and expressing in a given tissue is shown in Additional file 2: Table S1