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Fig. 6 | BMC Genomics

Fig. 6

From: MicroRNA and mRNA expression profiling analysis revealed the regulation of plant height in Gossypium hirsutum

Fig. 6

Validation of the expression patterns of the predicted DEGs and miRNAs by qRT-PCR and stem-loop qRT-PCR in dwarf mutant and tall-culm mutant. a 8 DEGs were validated by qRT-PCR in the mutants compared with that of wild type. b The expression patterns of five miRNAs were confirmed using stem-loop qRT-PCR. c Two miRNAs and their targets were validated using qRT-PCR and stem-loop qRT-PCR. The white bars represent the relative expression levels of stem-loop qRT-PCR or qRT-PCR. The equation ratio = 2Ct was applied to calculate the expression level relative to wild type Ari971, using GhUBQ7 as the reference gene. Three biological replicates and three technical replicates were performed. The black bars represent [Log2RPM A1 (A3)–A9] of data of sRNA-seq. A1: Ari1327, dwarf mutant; A3: Ari3697, tall-culm mutant, A9: wild type Ari971

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