An overview of the mitochondrial genome and transcriptome of Candida albicans.
a Schematic representation of the mitochondrial genome of C. albicans with known annotations. The major genomic regions: the short coding region (SCR), the inverted repeats (IRa and IRb), and the long coding region (LCR) are indicated. C. albicans mtDNA is mainly found as multiple head-to-tail concatamers, that result in an apparently circular restriction map, even though no actual circles are present . Protein coding genes are shown as green arrows, the ribosomal RNA genes (RNL and RNS) as light blue arrows, and tRNA genes as black triangles. Labels for tRNA genes were omitted for clarity. NAD6,1, etc., means two adjacent ORFS: NAD6 and NAD1 expressed from a bicistronic transcript. The major primary transcription units (TUs) are shown as red arrows, indicating direction of transcription. Inverted repeats are marked as shaded boxes. RNA-seq read coverage (based on all ~24 million pooled mapped reads) is displayed in its entirety (upper panel), and clipped at 100 reads to better visualize the expression of mRNA genes (bottom panel). Note that at this scale some short gaps between contiguous transcripts are not visible. b RNA-seq read coverage (clipped at 1000 reads) of the regions containing the transcription units. tRNA genes (black triangles) are labeled with one-letter codes of the respective amino acid. The separation of TU7 and TU8 is apparent only when read orientation is considered (inset), see also Fig. 6b