Fig. 2

Promoter sites in the C. albicans mitochondrial genome. a Sequencing reads near the transcription start sites of transcription units TU5 and TU6 (COX1 and COB, respectively). The conserved promoter nonanucleotides, and the start of the open reading frames are boxed. b Schematic representation of the mitochondrial genome of C. albicans with known genes, major genomic regions and transcription units, annotated as in Fig. 1. The second inverted repeat region (IRb) is identical to IRa in sequence, and was omitted for clarity. Putative promoter sites are marked by flags: larger red flags denote main promoters for transcription units, yellow flags correspond to putative additional promoters with some support from RNA-seq reads, and open flags are sequences conforming to the promoter consensus, but with no evidence for activity. Question marks indicate promoters that deviate significantly from the consensus sequence. Asterisks mark transcription start sites independently confirmed by 5’-RACE. c Sequences of the promoter sites depicted in (a), with the S. cerevisiae consensus nonanucleotide mitochondrial promoter and the T7 bacteriophage consensus promoter [88] included for comparison. d Sequence logo of the nonanucleotide promoters of the main transcription units (TU1-TU8), and e sequence logo of the first 5 nt of the primary transcripts. Logos created using WebLogo 3.4 [143]