TY - JOUR AU - Tsuda, Mai AU - Kaga, Akito AU - Anai, Toyoaki AU - Shimizu, Takehiko AU - Sayama, Takashi AU - Takagi, Kyoko AU - Machita, Kayo AU - Watanabe, Satoshi AU - Nishimura, Minoru AU - Yamada, Naohiro AU - Mori, Satomi AU - Sasaki, Harumi AU - Kanamori, Hiroyuki AU - Katayose, Yuichi AU - Ishimoto, Masao PY - 2015 DA - 2015/11/26 TI - Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing JO - BMC Genomics SP - 1014 VL - 16 IS - 1 AB - Functions of most genes predicted in the soybean genome have not been clarified. A mutant library with a high mutation density would be helpful for functional studies and for identification of novel alleles useful for breeding. Development of cost-effective and high-throughput protocols using next generation sequencing (NGS) technologies is expected to simplify the retrieval of mutants with mutations in genes of interest. SN - 1471-2164 UR - https://doi.org/10.1186/s12864-015-2079-y DO - 10.1186/s12864-015-2079-y ID - Tsuda2015 ER -