Table 5 Comparison of mutation detection by high resolution melting analysis and indexed amplicon sequencing
Gene locus | Length compared (bp) | All base changes obtained from two methods | Base changes common to both methods | HRM analysis only | Index amplicon sequencing only | Percentage of mutations detected by HRM analysis (%) | Percentage of mutations detected by index amplicon sequencing (%) |
|---|---|---|---|---|---|---|---|
Glyma20g25000 | 1144 | 37 | 14 | 4 | 19 | 48.6 | 89.2 |
Glyma08g46520 | 487 | 22 | 13 | 1 | 8 | 63.6 | 95.5 |
Glyma06g23026 | 305 | 12 | 7 | 0 | 5 | 58.3 | 100.0 |
Glyma20g22160 | 1187 | 24 | 12 | 4 | 8 | 66.7 | 83.3 |
Glyma11g15580 | 380 | 12 | 7 | 2 | 3 | 75.0 | 83.3 |
Total | 3503 | 107 | 53 | 11 | 43 | 62.5 | 90.3 |