Fig. 4

qRT-PCR validation of a selection of differentially expressed microRNAs. Validation was performed on independent samples from 14 patients. Significance of differential expression between tissue types was assessed using a paired t-test. *: significant modulation (p < 0.05) of expression between normal samples and tumors or LNM. **: significant modulation of expression between tumor samples and LNM. Relative quantification was calculated with the Pfaffl method and normalized between samples based on U6 SnRNA expression