Fig. 8

Three′ non-templated nucleotide additions and isomiRs distributions in PTC. a: the contribution of each individual addition was first computed for each mature microRNA in every of sample of the first sample set, and then averaged across all these samples. Normal samples, primary tumors and LNM from 3 patients were analyzed. Red dashed line corresponds to the minimum fraction of 1 % used to distinguish relevant additions from sequencing or trimming errors. b: only additions accounting for 1 % or more of all NTA in a given sample are represented. Other less frequent additions are grouped in the “other” category. Numbers correspond to the contribution, in percentage, of each type of addition averaged across all microRNAs having a minimum total mapped reads of 100. c/d/e: only isomiRs with a relative contribution to the total mapped reads of a mature microRNA of 1 % in at least one sample were considered. The number of isoforms per mature microRNA was then averaged by tissue type. The distributions were obtained in the 9 samples of the first sample set (c), in the TCGA samples dataset filtered based on clinical and pathological criteria (d) and in the complete TCGA dataset without additional filtering criteria (e). Numbers in brackets correspond to the number of available samples in the TCGA dataset for each tissue type. Eight available metastases samples were used. Independence of the distributions was assessed using a chi-squared test. The vast majority of microRNAs covered by a minimum of 100 reads have between 2 and 8 isomiRs