TY - JOUR AU - Luck, Ashley N. AU - Anderson, Kathryn G. AU - McClung, Colleen M. AU - VerBerkmoes, Nathan C. AU - Foster, Jeremy M. AU - Michalski, Michelle L. AU - Slatko, Barton E. PY - 2015 DA - 2015/11/11 TI - Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont JO - BMC Genomics SP - 920 VL - 16 IS - 1 AB - Filarial nematodes cause debilitating human diseases. While treatable, recent evidence suggests drug resistance is developing, necessitating the development of novel targets and new treatment options. Although transcriptomic and proteomic studies around the nematode life cycle have greatly enhanced our knowledge, whole organism approaches have not provided spatial resolution of gene expression, which can be gained by examining individual tissues. Generally, due to their small size, tissue dissection of human-infecting filarial nematodes remains extremely challenging. However, canine heartworm disease is caused by a closely related and much larger filarial nematode, Dirofilaria immitis. As with many other filarial nematodes, D. immitis contains Wolbachia, an obligate bacterial endosymbiont present in the hypodermis and developing oocytes within the uterus. Here, we describe the first concurrent tissue-specific transcriptomic and proteomic profiling of a filarial nematode (D. immitis) and its Wolbachia (wDi) in order to better understand tissue functions and identify tissue-specific antigens that may be used for the development of new diagnostic and therapeutic tools. SN - 1471-2164 UR - https://doi.org/10.1186/s12864-015-2083-2 DO - 10.1186/s12864-015-2083-2 ID - Luck2015 ER -