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Fig. 4 | BMC Genomics

Fig. 4

From: Molecular and phylogenetic characterization of the homoeologous EPSP Synthase genes of allohexaploid wheat, Triticum aestivum (L.)

Fig. 4

Mapping the chromosome locations of the wheat EPSPS genes using nulli-tetrasomic lines. PCR amplification was performed using indicated Chinese Spring nulli-tetrasomic genomic DNA. These lines contain no copy of one chromosome (nullisomic), and 4 doses (tetrasomic) of a homoeologous chromosome. The lines are referred to as nulli1A-tetra1D or N1A-T1D, N2A-T2D, etc. DNA was amplified with the indicated primer pairs for: a TaEPSPS-7D1 failed to amplify in N7D-T7B line with primers F18-D-R16-D indicating localization to chromosome 7D; (b and c) TaEPSPS-7A1 failed to amplify in N7A-T7D with indicated primers; (d and e) TaEPSPS-4A1 failed to amplify in N4A-T4D

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BMC Genomics

ISSN: 1471-2164

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