Fig. 3
From: DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs
Expression of gRNAs. a Primer design for specific detection of gRNAs by qRTPCR. The reverse primer is constant for all PCRs while a specific primer is designed for each gRNA. (b and c) Relative expression of transfected gRNAs in HCT116 cells (b) and Hela cells (c). Cells were transfected and selected with puromycin (2ug/mL) for 3 days. Data are normalised to the housekeeping gene GAPDH. Control amplifications were carried out using indicated primers with cDNA template from pDECKO-GFP cells