From: DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs
Targeted gene | Length of cut | Position to TSS | Target region | Expected PCR size (WT)(bp) | Expected PCR size (KO)(bp) |
---|---|---|---|---|---|
MALAT1_A | 100 bp | −70/+30 | Upstream TSS | 278 | 166 |
MALAT1_B | 500 bp | −400/+100 | Upstream TSS | 641 | 123 |
MALAT1_C | 600 bp | −430/+240 | Major TSS | 871 | 148 |
MALAT1_D | 600 bp | −450/+260 | Major TSS | 871 | 109 |
MALAT1_E | 3000 bp | −500/+2500 | Both TSS | 3249 | 224 |
UCA1 | 500 bp | −480/+160 | 819 | 181 | |
TFRC_A | 100 bp | −70/+30 | 215 | 97 | |
TFRC_B | 500 bp | −400/+100 | 637 | 141 | |
TFRC_C | 1000 bp | −700/+300 | 1240 | 239 |