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Table 1 Targeting constructs used in this study

From: DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs

Targeted gene Length of cut Position to TSS Target region Expected PCR size (WT)(bp) Expected PCR size (KO)(bp)
MALAT1_A 100 bp −70/+30 Upstream TSS 278 166
MALAT1_B 500 bp −400/+100 Upstream TSS 641 123
MALAT1_C 600 bp −430/+240 Major TSS 871 148
MALAT1_D 600 bp −450/+260 Major TSS 871 109
MALAT1_E 3000 bp −500/+2500 Both TSS 3249 224
UCA1 500 bp −480/+160   819 181
TFRC_A 100 bp −70/+30   215 97
TFRC_B 500 bp −400/+100   637 141
TFRC_C 1000 bp −700/+300   1240 239