First complete mitochondrial genome of the South American annual fish Austrolebias charrua (Cyprinodontiformes: Rivulidae): peculiar features among cyprinodontiforms mitogenomes

Table 3 Nucleotide variants in the mitogenomes of three full siblings of A. charrua

#position	Gene	Variant and total reads per individual			Nucleotide change	Ts or Tv	#codon	Codon change	Aminoacid change	Effect
#position	Gene	AUS4	AUS5	AUS7	Nucleotide change	Ts or Tv	#codon	Codon change	Aminoacid change	Effect
4105	ND2		2/167		C to A	Tv	22	TCG to TAG	Ser to STOP	Nonsense
5690	COI	2/74			T to A	Tv	75	ATT to AAA	Ile to Met	Non-synonymous
6245	COI	2/153	3/171		C to A	Tv	260	TAC to TAA	Tyr to STOP	Nonsense
7561	COII		2/109		G to T	Tv	131	GGC to GTC	Gly to Val	Non-synonymous
10220	ND3	2/151			A to G	Ts	109	CAA to CAG	Gln to Gln	Synonymous
11804	ND4	2/151			G to T	Tv	401	GGG to GGT	Gly to Gly	Synonymous
12505	ND5		2/191		T to C	Ts	103	TCT to CCT	Ser to Pro	Non-synonymous
12764	ND5		2/108		C to T	Ts	189	GCC to GCT	Ala to Ala	Synonymous
12962	ND5		2/85		G to T	Tv	255	AGC to ATC	Ser to Ile	Non-synonymous
15735	Cytb	44/93	35/90	15/53	T to C	Ts	368	TTT to CTT	Phe to Leu	Non-synonymous

Reads were mapped against the reference genome AUS7. The number of reads carrying the variant is shown together with total number the reads mapping to each position (variant reads/total reads)

ISSN: 1471-2164