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Fig. 5 | BMC Genomics

Fig. 5

From: Novel method to detect microRNAs using chip-based QuantStudio 3D digital PCR

Fig. 5

Absolute quantification of miRNAs in lung cancer cells. Bar graphs (left) show number of copies/μl of mir-16, mir-486 and mir-660 in H1299 (upper), A549 (middle) and LT73 (lower) cell lines and their respective ten-fold dilution. Representative dPCR plots of the miRNA analysis (right). The data points in the plot are color-coded according to the following call types: FAM (blue), VIC (red), FAM + VIC (green) and NOT AMPLIFIED (yellow)

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