Table 1 PCR run protocol
From: Novel method to detect microRNAs using chip-based QuantStudio 3D digital PCR
| Step type | Time | Temperature (°C) |
|---|---|---|
| Hold | 10 min | 96 |
| Cycle (40 cycles) | 2 min | 56 |
| 30 s | 98 | |
| Hold | 2 min | 60 |
| Hold | ∞ | 10 |
From: Novel method to detect microRNAs using chip-based QuantStudio 3D digital PCR
| Step type | Time | Temperature (°C) |
|---|---|---|
| Hold | 10 min | 96 |
| Cycle (40 cycles) | 2 min | 56 |
| 30 s | 98 | |
| Hold | 2 min | 60 |
| Hold | ∞ | 10 |