Fig. 1
From: Sequencing-based high throughput mutation detection in bread wheat
Schematic diagram for mutation detection using novel application of Genotyping-by-sequencing (GBS). a DNA of 24 plants including 22 EMS generated M2 and two wild types was digested with ApeKI followed by unique barcode (highlighted in orange, cyan, purple, and green) and common adapter (highlighted in red) ligation. All samples were pooled together before PCR amplification. Pooled sample library was evaluated for its quality and size. Sequencing of the library was performed on Illumina HiSeq2000. b Raw data files processed according to the filters described in Methods section. Differentiation of EMS SNPs from homoeologous SNPs is shown from three mutant plants where homoeologous SNP positions are marked in red and blue while mutational SNPs are highlighted in purple