Fig. 2

Plot of differential transcript abundance in Ribo-Zero and poly(A) transcriptomes. The y-axis shows log₂ fold-change of transcript abundance between Ribo-Zero and poly(A) samples. Greater than 0 indicates that a transcript is more abundant in Ribo-Zero samples than in poly(A) samples and vice versa, as indicated on the plot. Green lines indicate 1.5-fold change. Red points are significantly differentially represented among treatments (FDR-adjusted P-values < 0.05). Orange points are transcripts represented in only one sample, so fold-change could not be calculated. The x-axis shows the log₂ average abundance of the transcripts in both Ribo-Zero and poly(A) transcriptomes. The shape of the plot and distribution of significantly differentially represented transcripts indicates that low abundance transcripts show more variation among Ribo-Zero and poly(A) transcriptomes due to sampling effects than do higher abundance transcripts