Fig. 5

Example of putative novel transcript(s) represented in the Ribo-Zero libraries but not in the poly(A) libraries. The top panel shows a coverage depth summary plot (grey plot; log-scaled coverage depth) and aligned reads (red bars are reads from pairs aligned to the plus strand, blue bars from pairs aligned to the minus strand) for the poly(A) data. Below these are the same for the Ribo-Zero data. Below these are gene models (grey boxes represent exons, black lines un-translated regions: introns and UTRs) from the gene set AfunF1.2. A large transcribed region on the minus strand is evident in the Ribo-Zero data only, representing one or more putatively poly(A)- transcripts