Molecular tools for studying the major malaria vector Anopheles funestus: improving the utility of the genome using a comparative poly(A) and Ribo-Zero RNAseq analysis

Table 3 Summary of sense tag counting

Sample	Total tags^a	Assigned to features, sense orientation (%)^b	Assigned to non-rRNA features, sense orientation (%)^b,c	Assigned to features, antisense orientation (%)^b	Assigned to non-rRNA features, antisense orientation (%)^b,c
F1_XP_RZ	23,097,597	13,325,951 (57.69 %)	12,434,593 (53.84 %)	1,293,286 (5.60 %)	1,286,090 (5.57 %)
F1_XP_PA	24,317,689	16,120,214 (66.29 %)	13,404,217 (55.12 %)	1,234,142 (5.08 %)	1,216,317 (5.00 %)
F1_RZ	13,163,225	7,379,904 (56.06 %)	6,657,623 (50.58 %)	836,937 (6.36 %)	814,750 (6.19 %)
F1_PA	23,325,917	14,816,364 (63.52 %)	12,404,541 (53.18 %)	1,355,508 (5.81 %)	1,320,535 (5.66 %)
F2_RZ	14,787,076	8,421,844 (56.95 %)	8,063,710 (54.53 %)	1,006,847 (6.81 %)	1,001,013 (6.77 %)
F2_PA	21,970,208	14,411,931 (65.60 %)	12,390,885 (56.40 %)	1,237,662 (5.63 %)	1,218,099 (5.54 %)
F3_RZ	16,130,058	8,495,428 (52.67 %)	7,724,109 (47.89 %)	997,369 (6.18 %)	984,961 (6.11 %)
F3_PA	21,753,683	13,447,922 (61.82 %)	10,993,205 (50.53 %)	1,126,921 (5.18 %)	1,112,413 (5.11 %)

^aA tag is a read pair or a single, un-paired read
^b% of “Total tags”
^cTags assigned to features after subtracting tags assigned to 10 putative rRNA features

ISSN: 1471-2164