Fig. 6
From: De novo transcriptome reconstruction and annotation of the Egyptian rousette bat
Unannotated, novel transcripts from R. aegyptiacus were validated of by RT-PCR. RNA from the spleen of both bats was reverse transcribed to make cDNA. The cDNA was amplified using primers specific for one of 8 novel transcripts that were unannotated in the assembly, but contained a complete ORF larger than 400 nucleotides. The expected product sizes were: transcript 1, 457 bp; transcript 2, 450 bp; transcript 3, 419 bp; transcript 4, 548 bp; transcript 5, 469 bp, transcript 6, 277 bp; transcript 7, 507 bp; and transcript 8, 301 bp