Fig. 3

Genome wide scan for TUV86-2 susceptibility revealed a significant QTL on chromosome 9. a) A mouse chromosome interval map based on TUV86-2 colonization levels on day one. The x-axis depicts the physical map of the entire murine genome. The left y-axis illustrates the LRS (blue line) as an expression of strength of the association between colonization and genotypic markers. The grey line at y = 11.25 indicates the suggestive threshold, while the pink line at y = 17.88 shows the significant threshold. A significant QTL was identified on proximal Chr 9. b) Expanded physical map of Chr 9 for the region of the QTL. (a) Colored blocks represent the location of individual genes along the Chr. with links to corresponding section of the genome in the UCSC Genome Browser, the Ensembl Genome Browser, and expanded WebQTL map. (b) Haplotype map of BXD strains (listed on the left side with the log geometric mean colonization on day one listed next to the strain name), where green denotes D2 (paternal), red designates B6 (maternal), blue shows heterozygous and grey indicates an unknown genotype. Genetic markers associated with the mapped QTLs are shown: proximal mCV25073238, rs13480072, rs13480071, rs13480073, mCV24962297, gnf09.010.169 (associated with highest LRS), rs6183014, rs13480107, rs13480109 and CEL-9_29909656. (c) Expanded view of the QTL (blue line) overlaid on the SNP seismograph track, where each orange hash mark indicates a unique SNP. The right y-axis represents the additive allele effect and the red line signifies that the B6 allele is associated with increased colonization levels