Fig. 1

Identification and distribution of vasa-positive PGCs by whole mount in situ hybridization in WT (a-e) and dnd-MO injected (2000 pg/per embryo) (f-j) embryos from 50 % epiboly to 36 hpf. a and f 50 % epiboly stage, b and g bud stage, c and h 3-somite stage, d and i 24 hpf, and e and j 36 hpf. (Scale bar, 250 μm). k Average number of vasa-positive PGCs/per embryo in embryos injected with con-MO (circle) or dnd-MO (triangle) from sphere to 24 hpf. N ≥ 30, and the statistical data are presented as mean ± standard deviation (SD), P < 0.001