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Table 3 The role of mutations detected in R6-SOCPR in resistance to phage SOCP

From: A genomic approach to understand interactions between Streptococcus pneumoniae and its bacteriophages

Strainsa

Allelesb

EOPc

spr0058

spr0093

spr0897

spr1191

srp1443

spr1584

spr1777

spr1923

(SOCP)

R6

WT

WT

WT

WT

WT

WT

WT

WT

1

R6smR

WT

WT

WT

WT

WT

WT

WT

WT

1

R6-SOCPR

R

R

R

R

R

R

R

R

10−9

R6spr0058

R

WT

WT

WT

WT

WT

WT

WT

10−3

R6Δspr0058

noned

WT

WT

WT

WT

WT

WT

WT

10−3

R6spr0093

WT

R

WT

WT

WT

WT

WT

WT

1

R6spr0897

WT

WT

R

WT

WT

WT

WT

WT

10−3

R6spr1191

WT

WT

WT

R

WT

WT

WT

WT

1

R6spr1443

WT

WT

WT

WT

R

WT

WT

WT

10−3

R6spr1584

WT

WT

WT

WT

WT

R

WT

WT

1

R6spr1777

WT

WT

WT

WT

WT

WT

R

WT

1

R6spr1923

WT

WT

WT

WT

WT

WT

WT

R

1

R6spr0058-0897

R

WT

R

WT

WT

WT

WT

WT

10−9

R6spr0058-1443

R

WT

WT

WT

R

WT

WT

WT

10−9

R6spr0897-1443

WT

WT

R

WT

R

WT

WT

WT

10−9

R6spr0058-0897-1443

R

WT

R

WT

R

WT

WT

WT

10−9

R6-SOCPR_spr0058WT

WT

R

R

R

R

R

R

R

10−6

R6-SOCPR_spr0093WT

R

WT

R

R

R

R

R

R

1

R6-SOCPR_spr0897WT

R

R

WT

R

R

R

R

R

10−6

R6-SOCPR_spr1191WT

R

R

R

WT

R

R

R

R

1

R6-SOCPR_spr1443WT

R

R

R

R

WT

R

R

R

10−6

R6-SOCPR_spr1584WT

R

R

R

R

R

WT

R

R

1

R6-SOCPR_spr1777WT

R

R

R

R

R

R

WT

R

1

R6-SOCPR_spr1923WT

R

R

R

R

R

R

R

WT

1

  1. aR6smR integrated an rpsL allele conferring resistance to streptomycin (smR). Each mutated and WT alleles presented in the table was co-transformed with this rpsL allele that was used as a surrogate marker for the selection of transformants (see Methods). Although not indicated, every transformants in Table 3 are smR
  2. bWT, S. pneumoniae R6 WT gene sequence; R, S. pneumoniae R6-SOCPR gene sequence
  3. cEOP, efficiency of plaquing. Represents the ratio of phage titers from the test strain to the indicator strain R6 WT. Measured from three independent triplicates
  4. dThe gene spr0058 has been inactivated by insertion-duplication mutagenesis in this strain