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Table 5 Targeted screening for mutations in additional bacteriophage insensitive mutants

From: A genomic approach to understand interactions between Streptococcus pneumoniae and its bacteriophages

Strainsa

Allelesb,c

spr0058

spr0897

spr1130

spr1443

R6-SOCPR

G460A

C484A

NA

T1223C

E154K

P162T

V408A

R6-SOCP-2R

no mutation

no mutation

NA

no mutation

R6-SOCP-3R

no mutation

C485T

NA

T1223C

 

P162L

V408A

R6-SOCP-4R

C393A

no mutation

NA

T1223C

stop

V408A

R6-SOCP-5R

no mutation

no mutation

NA

T1223C

V408A

R6-DP1R

NA

NA

T985Gd

NA

G986Cd

C329A

R6-DP1-2R

NA

NA

T985Gd

NA

G986Cd

C329A

R6-DP1-3R

NA

NA

T985Gd

NA

G986Cd

C329A

R6-DP1-4R

NA

NA

no mutation

NA

R6-DP1-5R

NA

NA

T985Gd

NA

G986Cd

C329A

  1. aFive different mutants had initially been obtained from the same S. pneumoniae R6 parental culture for the selection of mutants insensitive to the phages SOCP or Dp-1
  2. bFor each mutation, the change at the nucleotide level is indicated on top and the corresponding substitution at the amino acid level is indicated in italic underneath
  3. cNA, not applicable. These alleles were not sequenced because they should be irrelevant to the resistance phenotype of the phage
  4. dBoth mutated positions are part of the same codon and lead to a single amino acid substitution