Fig. 1

Flowchart depicting the workflow for known miRNA profiling and novel miRNA prediction. The workflow comprises of four parts namely: Processing of the reads, Quantification of known miRNAs, the Elimination pipeline and the Novel miRNA prediction pipeline. For the elimination pipeline, the reads were matched with the known annotated genomic sequences. Alignment with maximum of two mismatches was considered as matched reads. All the matched reads were removed before the next round of elimination. The quantification of known miRNAs and prediction of novel miRNAs was done using miRDeep2 modules