Deep sequencing of small RNA facilitates tissue and sex associated microRNA discovery in zebrafish

BMC Genomics

Table 1 Details of the zebrafish sRNA deep sequencing datasets

Library	Replicates	Total number of reads	Total number of reads (in millions)	Percentage of reads mapped
Embryo	Embryo1	7,565,225	7.6	94.4
	Embryo2	10,202,969	10.2	94.8
	Embryo3	32,247,131	32.2	94.8
Male Brain	Male Brain1	5,101,418	5.1	97.8
	Male Brain2	6,533,205	6.5	97.3
	Male Brain3	10,518,347	10.5	97.0
Female Brain	Female Brain1	2,338,429	2.3	96.7
	Female Brain2	3,278,013	3.3	97.5
	Female Brain3	12,648,618	12.6	97.8
Male Gut	Male Gut1	16,232,210	16.2	97.1
	Male Gut2	8,864,108	8.9	97.7
	Male Gut3	3,117,877	3.1	95.8
Female Gut	Female Gut1	13,939,922	13.9	97.4
	Female Gut2	8,260,495	8.3	97.8
	Female Gut3	7,493,835	7.5	97.9
Male Liver	Male Liver1	18,404,267	18.4	97.8
	Male Liver2	9,369,428	9.4	97.4
	Male Liver3	4,639,550	4.6	97.1
Female Liver	Female Liver1	5,060,891	5.1	97.0
	Female Liver2	8,162,716	8.2	97.2
	Female Liver3	1,646,108	1.6	96.4
Ovary	Ovary1	8,824,267	8.8	95.8
	Ovary2	14,310,754	14.3	95.9
	Ovary3	25,898,221	25.9	95.9
Testis	Testis1	17,427,858	17.4	92.5
	Testis2	20,235,869	20.2	92.2
	Testis3	14,203,535	14.2	92.5
Eye	Eye1	9,398,588	9.4	96.0
	Eye2	5,722,393	5.7	95.5
	Eye3	2,854,316	2.9	95.9
Heart	Heart1	38,070,247	38.1	95.2
Heart	Heart2	34,828,065	34.8	96.2

Each tissue library comprised of three biological replicates. One of the replicate of the heart did not cluster well with the other replicates and was not considered for further analysis. The table shows the number of processed (adaptor trimmed, > = 18nt) reads for each biological replicate. Around 92–98% of reads mapped to the zebrafish genome

ISSN: 1471-2164