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Table 4 Summary of reads mapped to reference genes (de novo assembly of transcriptome)

From: RNA-seq reveals differentially expressed genes of rice (Oryza sativa) spikelet in response to temperature interacting with nitrogen at meiosis stage

Samples Total reads Total base pairs mapped reads Perfect match <=2 bp Mismatch Unique match Multi-position match Total unmapped reads
ZHH-1 12,532,019 (100.00 %) 614,068,931 (100.00 %) 10,688,571 (85.29 %) 9,100,428 (72.62 %) 1,588,143 (12.67 %) 9,065,210 (72.34 %) 1,623,361 (12.95 %) 1,843,448 (14.71 %)
ZHH-2 11,933,716 (100.00 %) 584,752,084 (100.00 %) 10,130,167 (84.89 %) 8,669,541 (72.65 %) 1,460,626 (12.24 %) 8,601,124 (72.07 %) 1,529,043 (12.81 %) 1,803,549 (15.11 %)
ZHN-1 12,029,191 (100.00 %) 589,430,359 (100.00 %) 10,194,826 (84.75 %) 8,731,597 (72.59 %) 1,463,229 (12.16 %) 8,703,059 (72.35 %) 1,491,767 (12.40 %) 1,834,365 (15.25 %)
ZHN-2 12,381,854 (100.00 %) 606,710,846 (100.00 %) 10,448,097 (84.38 %) 8,879,274 (71.71 %) 1,568,823 (12.67 %) 8,891,358 (71.81 %) 1,556,739 (12.57 %) 1,933,757 (15.62 %)
ZNH-1 12,404,809 (100.00 %) 607,835,641 (100.00 %) 10,518,064 (84.79 %) 9,018,788 (72.70 %) 1,499,276 (12.09 %) 8,850,183 (71.34 %) 1,667,881 (13.45 %) 1,886,745 (15.21 %)
ZNH-2 12,057,086 (100.00 %) 590,797,214 (100.00 %) 10,235,897 (84.90 %) 8,758,708 (72.64 %) 1,477,189 (12.25 %) 8,619,541 (71.49 %) 1,616,356 (13.41 %) 1,821,189 (15.10 %)
ZNN-1 12,245,417 (100.00 %) 600,025,433 (100.00 %) 10,454,895 (85.38 %) 8,914,987 (72.80 %) 1,539,908 (12.58 %) 8,797,125 (71.84 %) 1,657,770 (13.54 %) 1,790,522 (14.62 %)
ZNN-2 11,936,315 (100.00 %) 584,879,435 (100.00 %) 10,150,654 (85.04 %) 8,632,637 (72.32 %) 1,518,017 (12.72 %) 8,601,628 (72.06 %) 1,549,026 (12.98 %) 1,785,661 (14.96 %)
  1. Note: NH, Normal nitrogen level and high temperature treatment; NN, Normal nitrogen level and natural temperature treatment; HH, High nitrogen level and high temperature treatment; HN, High nitrogen level and natural temperature treatment. “Z” represents Zhong 531, 4 treatment combinations and 2 pooling duplicates. The same as follows