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Table 4 Summary of reads mapped to reference genes (de novo assembly of transcriptome)

From: RNA-seq reveals differentially expressed genes of rice (Oryza sativa) spikelet in response to temperature interacting with nitrogen at meiosis stage

Samples

Total reads

Total base pairs

mapped reads

Perfect match

<=2 bp Mismatch

Unique match

Multi-position match

Total unmapped reads

ZHH-1

12,532,019 (100.00 %)

614,068,931 (100.00 %)

10,688,571 (85.29 %)

9,100,428 (72.62 %)

1,588,143 (12.67 %)

9,065,210 (72.34 %)

1,623,361 (12.95 %)

1,843,448 (14.71 %)

ZHH-2

11,933,716 (100.00 %)

584,752,084 (100.00 %)

10,130,167 (84.89 %)

8,669,541 (72.65 %)

1,460,626 (12.24 %)

8,601,124 (72.07 %)

1,529,043 (12.81 %)

1,803,549 (15.11 %)

ZHN-1

12,029,191 (100.00 %)

589,430,359 (100.00 %)

10,194,826 (84.75 %)

8,731,597 (72.59 %)

1,463,229 (12.16 %)

8,703,059 (72.35 %)

1,491,767 (12.40 %)

1,834,365 (15.25 %)

ZHN-2

12,381,854 (100.00 %)

606,710,846 (100.00 %)

10,448,097 (84.38 %)

8,879,274 (71.71 %)

1,568,823 (12.67 %)

8,891,358 (71.81 %)

1,556,739 (12.57 %)

1,933,757 (15.62 %)

ZNH-1

12,404,809 (100.00 %)

607,835,641 (100.00 %)

10,518,064 (84.79 %)

9,018,788 (72.70 %)

1,499,276 (12.09 %)

8,850,183 (71.34 %)

1,667,881 (13.45 %)

1,886,745 (15.21 %)

ZNH-2

12,057,086 (100.00 %)

590,797,214 (100.00 %)

10,235,897 (84.90 %)

8,758,708 (72.64 %)

1,477,189 (12.25 %)

8,619,541 (71.49 %)

1,616,356 (13.41 %)

1,821,189 (15.10 %)

ZNN-1

12,245,417 (100.00 %)

600,025,433 (100.00 %)

10,454,895 (85.38 %)

8,914,987 (72.80 %)

1,539,908 (12.58 %)

8,797,125 (71.84 %)

1,657,770 (13.54 %)

1,790,522 (14.62 %)

ZNN-2

11,936,315 (100.00 %)

584,879,435 (100.00 %)

10,150,654 (85.04 %)

8,632,637 (72.32 %)

1,518,017 (12.72 %)

8,601,628 (72.06 %)

1,549,026 (12.98 %)

1,785,661 (14.96 %)

  1. Note: NH, Normal nitrogen level and high temperature treatment; NN, Normal nitrogen level and natural temperature treatment; HH, High nitrogen level and high temperature treatment; HN, High nitrogen level and natural temperature treatment. “Z” represents Zhong 531, 4 treatment combinations and 2 pooling duplicates. The same as follows