Fig. 3

Global expression patterns. a Heatmap of target genes and all gonad samples. Gene names are shown in the bottom of the figure while gene hierarchical cluster is shown in the top. Log fold change expression values representation ranges from red (highest expression) to light green (lowest expression). Sample names are not shown, instead each sample is represented by the three colors at the left of the figure which indicate sex (magenta for females, and blue for males), age (ranging from 60 to 135 dpf corresponding to a scale going from grey to dark green) and temperature (light blue for 15 °C, yellow for 18 °C and red for 23 °C). Yellow, black or blue circles highlight expression patterns characteristic of undifferentiated, female or male individuals respectively. b Samples were grouped according to the fold change expression values of the target genes by a principal component analysis. Samples labeled as “Female” and “Male” and colored in red and purple, respectively, represent gonad samples which were both genetically and histologically sexed because developing testis and ovaries could be distinguished from each other. Samples labeled as “GenFemale” and “GenMale” and colored in olive green and light blue, respectively, are gonad samples which were only genetically sexed because histologically the gonads were still undifferentiated. A 66 % of the samples of each group are expected to be placed in their respective circles. The arrows with the name of the genes at the end represent how each gene contributes to the two principal analysis components represented in the figure