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Fig. 1 | BMC Genomics

Fig. 1

From: Fast evolutionary rates associated with functional loss in class I glucose transporters of Schistosoma mansoni

Fig. 1

Relative expression of sgtp1, sgtp2, sgtp3 and sgtp4 after transformation and adding glucose. The relative expression of sgtp1, sgtp4 (Panel a), sgtp2 and sgtp3 (Panel b) in schistosomula was determined using quantitative RT-PCR. Cercariae were mechanically transformed into schistosomula. A total of 10 000 schistosomula were incubated for 3 h with traces of glucose (0.05 mM). Subsequently, glucose, maltose and galactose were added to a final concentration of 10 mM. After 8 h of incubation, total RNA was extracted, cDNA was synthesized and quantitative RT-PCR was performed. Transcripts of sgtp1 (p-value = 0.0006) and sgtp4 (p-value = 0.02) were significantly downregulated after adding 10 mM glucose, while sgtp2 (p-value = 0.002) and sgtp3 (p-value = 0.02) were significantly upregulated after adding 10 mM glucose. Results shown are the means of three independent experiments. Gene expression data is presented relative to 8 h with 0.05 mM of glucose which was used as “calibrator sample”

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