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Table 2 Structural and subcellular localization analysis of the HbAQPs

From: Genome-wide identification of rubber tree (Hevea brasiliensis Muell. Arg.) aquaporin genes and their response to ethephon stimulation in the laticifer, a rubber-producing tissue

Name Len Mw (KDa) pI TMa TMb TMc Locd Ar/R selectivity filter NPA motifs Froger's positions
H2 H5 LE1 LE2 LB LE PI P2 P3 P4 P5
HbPIP1;1 287 30.80 8.59 6 6 6 Plas F H T R NPA NPA E S A F W
HbPIP1;2 287 30.80 8.59 6 6 6 Plas F H T R NPA NPA E S A F W
HbPIP1;3 287 30.74 8.24 6 6 6 Plas F H T R NPA NPA E S A F W
HbPIP1;4 287 30.78 8.62 6 6 6 Plas F H T R NPA NPA E S A F W
HbPIP1;5 269 28.92 8.60 5 6 5 Plas F H T R NPA NPA Q S A F W
HbPIP2;1 288 30.71 7.61 6 6 6 Plas F H T R NPA NPA Q S A F W
HbPIP2;2 288 30.71 8.20 6 6 6 Plas F H T R NPA NPA Q S A F W
HbPIP2;3 286 30.58 8.50 6 6 6 Plas F H T R NPA NPA Q S A F W
HbPIP2;4 286 30.61 8.19 6 6 6 Plas F H T R NPA NPA Q S A F W
HbPIP2;5 285 30.34 9.18 6 6 6 Plas F H T R NPA NPA Q S A F W
HbPIP2;6 286 30.39 9.06 6 6 6 Plas F H T R NPA NPA Q S A F W
HbPIP2;7 278 29.56 9.11 6 6 6 Plas F H T R NPA NPA M S A F W
HbPIP2;8 280 29.76 8.97 6 6 6 Plas F H T R NPA NPA M S A F W
HbPIP2;9 280 29.84 6.51 6 6 6 Plas F H T R NPA NPA M S A F W
HbPIP2;10 285 30.45 9.05 6 6 6 Plas F H T R NPA NPA M S A F W
HbTIP1;1 252 25.91 5.91 7 6 7 Vacu H I A V NPA NPA T S A Y W
HbTIP1;2 252 26.07 5.70 7 6 7 Vacu H I A V NPA NPA T S A Y W
HbTIP1;3 253 26.44 6.27 6 6 6 Cyto H I A V NPA NPA T S A Y W
HbTIP1;4 227 23.78 6.18 3 6 4 Vacu H I A V NPA NPA T S A Y W
HbTIP1;5 252 25.88 4.96 7 6 6 Vacu/Cyto H I A V NPA NPA T S A Y W
HbTIP1;6 252 25.79 5.70 7 6 7 Cyto H I A V NPA NPA T S A Y W
HbTIP1;7 252 25.90 4.97 6 6 6 Vacu/Cyto H I A V NPA NPA T S A Y W
HbTIP1;8 252 25.72 4.79 6 7 6 Vacu/Cyto H I A V NPA NPA T S A Y W
HbTIP2;1 248 25.40 5.33 7 6 6 Vacu H I G R NPA NPA T S A Y W
HbTIP2;2 248 25.34 5.59 7 6 7 Vacu H I G R NPA NPA T S A Y W
HbTIP2;3 250 25.31 4.87 6 6 6 Vacu H i G R NPA NPA T S A Y W
HbTIP2;4 250 25.31 4.59 4 6 5 Vacu/Plas H I G S NPA NPA A S A Y W
HbTIP3;1 257 27.38 6.43 6 6 6 Cyto H I A R NPA NPA T A A Y W
HbTIP3;2 243 25.66 9.74 6 6 6 Cyto H I A R NPA NPA T A A Y W
HbTIP4;1 251 26.24 5.91 6 6 7 Vacu H I A R NPA NPA T S A Y W
HbTIP5;1 252 25.95 6.71 5 6 6 ER N V G C NPA NPA I A A Y W
HbTIP5;2 247 25.32 5.13 6 6 6 Chlo N V G C NPA NPA I A A Y W
HbNIP1;1 286 30.41 7.57 6 6 6 Plas W V A R NPA NPA F S A Y L
HbNIP1;2 287 30.39 8.94 6 6 6 Plas W V A R NPA NPA F S A Y I
HbNIP2;1 285 29.88 8.72 6 6 6 Vacu G S G R NPA NPA L S A Y I
HbNIP3;1 282 30.20 8.34 6 6 5 Chlo W V A R NPA NPA F S A F I
HbNIP4;1 267 28.80 5.28 6 6 6 Plas W V G R NPA NPV L S A Y I
HbNIP4;2 281 29.60 5.71 6 6 6 Plas W V A R NPA NPA F S A Y I
HbNIP5;1 298 30.98 8.65 6 6 5 Plas A I G R NPS NPV F T A Y L
HbNIP6;1 305 31.58 8.68 6 6 5 Plas T I A R NPS NPV F T A Y L
HbNIP7;1 298 31.85 6.50 6 6 6 Plas A V G R NPA NPA Y S A Y I
HbSIP1;1 239 25.35 9.54 5 6 5 Vacu A V P N NPT NPA M A A Y W
HbSIP1;2 239 25.97 7.74 6 6 6 Plas F V P N NPT NPA M A A Y W
HbSIP1;3 239 25.83 9.36 6 6 5 Plas V V P N NPT NPA M A A Y W
HbSIP2;1 240 26.40 9.60 4 6 5 Chlo S H G S I NPl NPA F V A Y W
HbXIP1;1 289 31.23 6.36 6 6 6 Cyto V V V R SPV NPA M C A F W
HbXIP1;2 296 32.13 8.06 6 7 6 Chlo V V V R SPI NPA M F A F W
HbXIP1;3 276 29.63 7.50 5 6 5 Cyto V I P R NPT NPA M C A F W
HbXIP1;4 276 29.50 8.31 5 6 5 Vacu V I A R NPT NPA M C A F W
HbXIP2;1 305 32.28 8.61 6 6 6 Plas   V V R NPV NPA V C A F W
HbXIP3;1 256 27.16 8.87 6 7 5 Vacu V V A R NPL NPA V C A F W
  1. a,b,cRepresenting the numbers of transmembrane helices predicted by TOPCONS, TMPRED and TMHMM, respectively; dBest possible subcellular localization prediction by the WoLF PSORT. (Ar/R aromatic/arginine, Chlo chloroplast, Cyto cytosol, ER endoplasmic reticulum, H2 transmembrane helix 2, H5 transmembrane helix 5, LE loop E, Loc subcellular localization, NPA Asn-Pro-Ala, Plas plasma membrane, TM transmembrane helix, Vacu vacuolar membrane)