Fig. 5

Phylogenetic analysis of P. ulmi ABC protein NBDs. Maximum likelihood midpoint rooted tree of N- and/or C-terminal NBDs extracted from P. ulmi and P. citri ABC protein coding ORFs and T. urticae and D. melanogaster ABC protein sequences. The scale bar represents 0.5 amino-acid substitutions per site. P. ulmi and P. citri NBDs clustered within the eight currently described ABC subfamilies (a to h). The tree was midpoint rooted and numbers at the branch point of each node represent the bootstrap value resulting from 1000 pseudoreplicates (LR-ELW). C-terminal NBDs of ABCC transporters clustered together with NBDs of ABCB transporters [57]. Colour and shape codes are as follows: P. ulmi, green triangle, P. citri, yellow triangle, T. urticae, red square and D. melanogaster, black dot. ABC protein sequences and accession numbers can be found in Additional file 11