Fig. 1

Experimental design and results of Neurospora telomere pull-down. a Schematic workflow of the label-free telomere interaction screen. Extracts of N. crassa were incubated with either a telomeric probe (TTAGGG) or a shuffled control sequence (TGTGAG). For each probe, four pull-downs were performed independently and measured using high resolution mass spectrometry. To identify TTAGGG repeat binding proteins the MaxLFQ algorithm was used to compare individual peptide intensities between the telomeric bait and the shuffled control sequence. b Volcano plot of the telosome of N. crassa. We identified 12 proteins (two-sided t-test, Welch, FDR = 0.05) to be enriched at the telomeric TTAGGG probe compared to the shuffled control. c Heatmap representation of z-scored enrichment values obtained from MaxLFQ intensities for the 12 significantly enriched Neurospora proteins validates reproducible binding behaviour in each replicate