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Fig. 3 | BMC Genomics

Fig. 3

From: Genome-wide miR-155 and miR-802 target gene identification in the hippocampus of Ts65Dn Down syndrome mouse model by miRNA sponges

Fig. 3

Decoy activity of Lv-miR155-802T significantly reduces hippocampal miR-155 and miR-802 content in Ts65Dn mice. a Schematic representation of the experimental design for local lentiviral infusion and sample collection. b Expression analysis by RT-qPCR (Applied Biosystems) of mmu-miR-155 (left panel) and mmu-miR-802 (right panel) in RNA extracts from hippocampus of euploid (EU) and Ts65Dn mice treated with Lv-Control or Lv-miR155-802T. RQ values represent mean ± SEM of six independent samples; *p < 0.05 and **p < 0.01 (Kruskal-Wallis test). c Expression analysis by RT-qPCR (Roche) of mmu-miR-155 and mmu-miR-802 validated target gene Mecp2, the mmu-miR-155 target Ship1 and the mmu-miR-802 validated target FoxM1 in RNA extracts from hippocampus of euploid (EU) and trisomic (Ts65Dn) adult mice treated with Lv-Control or Lv-miR155-802T. Values represent mean ± SEM of six independent samples; ns, non significant and * p < 0.05 and ** p < 0.01 (Kruskal-Wallis test)

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