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Fig. 5 | BMC Genomics

Fig. 5

From: Genome-wide miR-155 and miR-802 target gene identification in the hippocampus of Ts65Dn Down syndrome mouse model by miRNA sponges

Fig. 5

Validation of novel target genes contributing to T565Dn phenotype by RT-PCR. a Upper panel shows a table with five predicted target genes selected based on the criteria of predicted number of target sites and strong correlation coefficient (Rufy2, Nova1, Nav1, Thoc1, Sumo3). Table shows correlation values and the number of predicted miRNA target sites classified according its evolutionary conservancy. Below panel shows the correlation plots calculated using miRComb package for the previously selected target genes. b Expression analysis by RT-qPCR (Roche) of miR-155 and miR-802 target genes in RNA extracts from hippocampus of euploid (EU) and trisomic (Ts65Dn) adult mice treated with Lv-Control or Lv-miR155-802T. Data normalized to the expression of GdX (also named Ubl4A) housekeeping gene. Values represent mean ± SEM of six independent samples. * and ns denote p < 0.05 and not significant, respectively (Kruskal-Wallis test)

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BMC Genomics

ISSN: 1471-2164

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