Fig. 1From: Transcription analysis of neonicotinoid resistance in Mediterranean (MED) populations of B. tabaci reveal novel cytochrome P450s, but no nAChR mutations associated with the phenotypePhylogenetic analysis of B. tabaci nAChR subunits. Maximum likelihood phylogenetic tree of B. tabaci Med (BtMed, indicated in red) and nAChR subunit protein sequences from 7 species: D. Melanogaster (Dmel), A. mellifera (Amel), T. castaneum (Tcas), A. gambiae (Agamb), B. mori (Bmori), A. pisum (Apisum), B. tabaci (Btab). Sequences were aligned using mafft and a bootstrapped midpoint-rooted tree was constructed (1000 bootstraps). Bootstrap percentage is indicated for crucial branches. For accession numbers of insects nAChR subunit protein sequences see Additional file 5: Figure S2Back to article page