Fig. 1
From: Genome-wide mapping of Hif-1α binding sites in zebrafish
vhl mutants shows specific enrichment for the epo-HRE. Graph showing the result of a ChIP-qPCR experiment. In vhl mutants HIF is stabilised and is expected to bind to HREs. Strong enrichment (22-fold) for the region spanning the epo-HRE is seen in vhl mutants, whereas the wild-type HRE, and flanking regions both up- and down-stream of the HRE are much less enriched. A control region ~2Kb distally was used for comparison. Details for the sequences and relative positions of primers as well as the fold change calculation can be found in the Additional file 14 and the Methods section