Skip to main content
Fig. 5 | BMC Genomics

Fig. 5

From: Expression of microRNAs and isomiRs in the porcine endometrium: implications for gene regulation at the maternal-conceptus interface

Fig. 5

isomiRs work cooperatively with canonical miRNAs to affect putative targets gene expression in endometrial stromal cells. a Venn diagrams demonstrating relations between targets identified for canonical and non-canonical miR-140-3p harboring different seed sequences. b Top functional annotations organized into network representing common target mRNAs regulated by canonical and non-canonical miR-140-3p. The network is displayed graphically as nodes (genes/genes products) and edges (biological relationship between nodes). The shapes of nodes indicate the functional class of the gene product, explained in the legend. All connections were integrated into the computationally generated networks on the basis of the evidence stored in the IPA knowledge memory indicating relevance for these networks. c Fold change expression of miR-140-3p and its 5’ isomiR mimic transfected into endometrial stromal cells collected on D16 of pregnancy (n = 6) is compared to negative control. let-7d-3p was used as reference for miR-140-3p and isomiR miR-140-3p 5’ DEL U expression. Mimics were used at 50 nM dose when added separately or 25 nM dose each when added simultaneously. d Relative expression of canonical and non-canonical miR-140-3p putative target genes after mimics transfection into endometrial stromal cells from D16 of pregnancy (n = 4-6). Levels of ACVR2B, KCMA1, SIRT1, LIF and LPAR2 genes are presented relatively to geometric means of HPRT1 and ACTB. Results are shown as boxplots with means ± SEM. Asterisks indicate significant differences, in comparison to the negative control (50 nM; miRNA negative control mimic). *p < 0.05; **p < 0.01; ***p < 0.001

Back to article page