Fig. 4

The seasonal miR-132–mRNA regulatory network in HVC. a The relative expression changes of mRNA targets of miR-132 that were differentially regulated in at least one experimental group (i.e. LD + T 3D, 21, or LDW) compared to SD, as well as having an inverse correlation to miR-132 in the same comparison. A p-value of <0.005 and a fold change >1.5 at any time point were used as selection criteria for mRNAs presented in the heat map. b An interaction network of mRNAs that were differentially anti-expressed with a fold change > -1.5 and p < 0.005 from conditions in which miR-132 was also differentially expressed (i.e. fold change > 2.0). IPA network analyses revealed several key pathways were down-regulated during periods of HVC new neuronal addition and functional incorporation