Fig. 4

Transcriptional regulator DVU2956, and not DVU2960, binds upstream of target gene DVU2957. Lanes 1–5 show electrophoretic mobility shift assays with full-length (370 bp) upstream region of DVU2957: lane 1 – DNA only; lanes 2 and 3 – with purified DVU2956 protein (260 and 52 pmol, respectively); lanes 4 and 5 – with purified DVU2960 protein (400 and 80 pmol, respectively). Lanes 6–11 show EMSAs with predicted binding site motif upstream of DVU2957 (wild-type motif –lanes 6–8; mutated motif – lanes 9–11). Sequences of the two sites are shown below the gel image; the three conserved half-sites are shown in caps, and substitutions made in the mutated site are shown in red. Lanes 6, 9 – DNA only; lanes 7, 10 – with 390 pmol of DVU2956 protein; lanes 8, 11 – with 260 pmol of DVU2956 protein