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Table 4 Proteomic analysis of haustoria and hyphae samples for experimental identification of EKA proteins.

From: Evolution of the EKA family of powdery mildew avirulence-effector genes from the ORF 1 of a LINE retrotransposon

 

Haustoria only

Hyphae only

Haustoria and hyphae

Proteins identified

AVRK1-like

Full length ORF1

0

3

0

Truncated (putative AVR/effectors)

0

1

0

Total

0

4

0

AVRA10-like

Full length ORF1

6

5

1

Truncated (putative AVR/effectors)

8

0

3

Total

14

5

4

Similar to both AVRK1/AVRA10

Full length ORF1

1

4

0

Truncated (putative AVR/effectors)

0

1

0

Total

1

5

0

Total proteins identified

15

14

4

Total peptides

15

21

1

 

ATVPALPR

AGLGRSAGPSITK

AAAPLPLR

 

DDRIFLRa

AHFHPSTR

 
 

GLESNIQNMNAIAAALLAKENDVPEVDMVDAEVEK

APKKEAPK

 
 

KASGPAETSR

ARPSKSGPVK

 
 

KPTPPTKKSPK

ASDLVALR

 
 

MLELSQTK

ASIAQFIQAGPGATPPVLPK

 
 

MPTPPTKQSAK

DQLVTIR

 
 

MPTPPTKR

EKTIEPAENSTRK

 
 

NAVSGTAKNR

ELLDSSTSRSVSGIK

 
 

NMPEVDMVDAEVEKLK

EPPNQPTPVMVSRAPK

 
 

RATVPALPR

FLPSLPQRQATQGK

 
 

SPKTATLSSPDNR

KMESDMTER

 
 

SWAALFPR

KREELLDAAPK

 
 

TLSPFGGR

LFQMPPTR

 
 

VPTSFALR

MPTPPSKKLAK

 
  

QGIPPGSIER

 
  

SDSRSCKSRPNK

 
  

SKKSGPVTK

 
  

SLAHKAAETDK

 
  

SWAALFPRNASPKPGFR

 
  

VPTGFTLR

 
  1. aPeptide DDRIFLR is just below the identity score threshold.