Fig. 2

Identification of differentially expressed genes (DEGs) in a male sterile line (DAH3615-MS) and a male fertile line (DAH3615). (a) Venn-diagram of significantly different DEGs detected in the male fertile line and the male sterile line using two types of statistical tests such as Fisher’s exact test and 2-way ANODEV [False discovery rate (FDR) adjusted P-value < 0.01]. In the Fisher’s exact test, two tissues’ samples, bud and floral bud, were respectively employed. (b) Hierarchical clustering analysis tree with k = 2. Blue and green lines represent DAH3615-MS and DAH3615, respectively. (c) Heatmap with hierarchical clustering using logged TMM-normalized gene expressions of the 1259 DEGs (FDR adjusted P-value < 0.01) from the analysis of deviance (ANODEV). Regions 1 and 2 show highly significant differences between DAH3615-MS and DAH3615. (d) Line plots show the gene expression of the top 10 genes from ANODEV. Red and blue lines represent floral bud and flower, respectively. The y-axis represents logged TMM-normalized gene expressions, which were calculated in the edgeR