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Table 3 qRT-PCR test results of 19 genes detected by RNA-sequencing

From: Transcriptome profiling of differentially expressed genes in floral buds and flowers of male sterile and fertile lines in watermelon

Gene description

Uniport_ID

Floral Bud

Flower

Actin depolymerizing factor 10

B9I489_POPTR

1.35E-04***

2.44E–03**

Arabinogalactan peptide 20

AGP20_ARATH

8.00E–04***

2.10E–04***

Blue copper protein (Fragment)

O82576_MAIZE

1.87E–04***

1.56E–03**

Calcium–dependent protein kinase 2

Q3YAT0_PETIN

1.10E–04***

4.03E–04***

Callose synthase 5

C8C9X3_ARATH

8.66E–05***

2.26E–02*

CBL–interacting protein kinase 16

C4P7W5_VITVI

2.92E–04***

1.92E–04***

Cyclin–like protein

Q6ZIX9_ORYSJ

2.59E–05***

6.36E–04***

Elongation factor 1–alpha

B9SPV9_RICCO

2.75E–03**

7.44E–04***

Late embryogenesis abundant protein 1

LEA1_CICAR

1.41E–04***

2.35E–04***

LIM domain protein

Q306K1_BRANA

8.75E–04***

5.13E–06***

MADS–box transcription factor 1

D1MFS6_HEVBR

1.99E–03**

6.63E–05***

Mitotic spindle assembly checkpoint protein MAD2

D2V3A0_NAEGR

5.34E–06***

8.07E–03**

Nucleotide sugar epimerase

B1WNM2_CYAA5

1.02E–02*

8.12E–04***

Pectate lyase

B6TSP4_MAIZE

7.71E–03**

2.84E–04***

Polygalacturonase

E3VSV7_CUCPE

1.42E–03**

1.96E–04***

Rho GDP–dissociation inhibitor family protein

D7L724_ARALL

4.51E–03**

9.35E–04***

S–adenosylmethionine synthase

B9S0G1_RICCO

2.14E–03**

6.32E–04***

Subtilisin–like protease

Q6WNU4_SOYBN

1.86E–02*

1.93E–02*

Zinc finger family protein

D7MD22_ARALL

3.19E–03**

5.41E–04***

  1. Student’s t–test was used for separating the differences between a male sterile line (DAH3615–MS) and a male fertile line (DAH3615) in floral buds and flowers. *P < 0.05, **P < 0.01, ***P < 0.001