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Table 2 Experimental conditions assessed in this study on IT, MS, 454 FLX + and PB sequencing platforms

From: A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling

Synthetic community

Experimental design

Nm cycles

Template concentration (ng)

Sequencing platform

EM/UM

Fusion primer

25

1,5,10

MS

EM

Fusion primer

25

2,5

IT, 454

EM

Universal tailed tag

5+15

1,10

MS

EM

Universal tailed tag

8+15

1,10

MS

EM/UM

Universal tailed tag

10+15

2,5

MS

EM/UM

Adapter ligation

25

500–750

PB